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ACLAR 33C
is a flexible thermoplastic fluoropolymer film with high optical clarity and a smooth surface. These features provide the basis for some novel solutions to specific embedding problems.
For
studies utilizing horseradish peroxidase (HRP), ACLAR has been successfully used to aid in light microscopy evaluation prior to thin sectioning. After processing through the standard HRP, fixation, dehydration and
embedding schedules, a slice of tissue sample with a drop of epoxy resin is placed on a small sheet of ACLAR. A second sheet of ACLAR is placed over the specimen and these two sheets are pressed between the area of
interest can be easily cut out and attached by "super glue" to a blank epoxy cylinder for ultramicrotomy. The ACLAR serves to keep the sections flat, providces excellent transparency and can be easily peeled from the
cured epoxies.
Macrophages selectively adhere to the surface of ACLAR while lymphocytes do not. After washing, an almost pure collection of macrophages can be produced using ACLAR disks as
substrates in standard tissue culture wells. The disks containing macrophages are then fixed, dehydrated, critical point dried and attached to SEM specimen mounts. Following a conductive evaporative coating, the
specimen can be examined at up to 20-25kV in the SEM. REF: "Ultrastructure of HRP-Labelled Neurons: A Comparison of Two Sensitive Techniques." G.M. Mawe, J.C. Bresnahan and M.S.
Beattie. Brain Research Bulletin,
10:551-558, 1983. |
