51290 - ACLAR Embedding Film, 8" x 10" x 7.8mil Thick, pkg 10

Quick Overview

ACLAR Embedding Film:

8" x 10" x 7.8 mil thick. 10 sheets/pkg


sku: 51290

Availability: In stock

ACLAR 33C is a flexible thermoplastic fluoropolymer film with high optical clarity and a smooth surface. These features provide the basis for some novel solutions to specific embedding problems. For studies utilizing horseradish peroxidase (HRP), ACLAR has been successfully used to aid in light microscopy evaluation prior to thin sectioning. After processing through the standard HRP, fixation, dehydration and embedding schedules, a slice of tissue sample with a drop of epoxy resin is placed on a small sheet of ACLAR. A second sheet of ACLAR is placed over the specimen and these two sheets are pressed between the area of interest can be easily cut out and attached by "super glue" to a blank epoxy cylinder for ultramicrotomy. The ACLAR serves to keep the sections flat, providces excellent transparency and can be easily peeled from the cured epoxies. Macrophages selectively adhere to the surface of ACLAR while lymphocytes do not. After washing, an almost pure collection of macrophages can be produced using ACLAR disks as substrates in standard tissue culture wells. The disks containing macrophages are then fixed, dehydrated, critical point dried and attached to SEM specimen mounts. Following a conductive evaporative coating, the specimen can be examined at up to 20-25kV in the SEM. REF: "Ultrastructure of HRP-Labelled Neurons: A Comparison of Two Sensitive Techniques." G.M. Mawe, J.C. Bresnahan and M.S. Beattie. Brain Research Bulletin, 10:551-558, 1983.
SKU 51290
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